The range of ROTI^®Antibiotic Discs is designed for the qualitative analysis of the sensitivity of bacteria or fungi to anti-microbiological reagents by means of in vitro diffusion methods.

A fresh starter culture is adjusted at a cell density of 1×10⁶-5×10⁶ cfu/ml (in acc. with McFarland Standard 0,5) and applied evenly to a culture medium plate with Mueller-Hinton agar (Art. No. X926; for fungal cultures add 2 % glucose + 0,5 μm methylene blue). The antibiotic discs are individually applied to the plate using sterile tweezers or a dispenser (Art. No. 1505).

After a suitable period of incubation, the size of the inhibiting zone around the discs is measured. The diameter of the inhibiting zone is assessed using standardised values in the available standards and databases and is an indicator of the culture’s sensitivity to the antibiotic.

Each ROTI^®Antibiotic Discs streptomycin (S) disc contains streptomycin at a concentration of 10 μg and has „S 10“ printed on both sides. The diameter of the ROTI^®Antibiotic Discs is 6 mm.

Tested according to CLSI.