Column-free, ultra-flexible isolation system designed for reliable isolation of total RNA from almost any tissue.The ROTI^®Quick Kit is suitable for use with cell culture material, biopsies, high-fat or high-protein tissue, blood (Üçeyler et al. Neurology (2007) 69:42-9), solid plant material, mitochondria (Backert et al. Plant Molecular Biology (1997) 33:1037-50), mycoplasms (Weiner et al. Nucleic Acids Res. (2000) 28:4488-96) and many more. The kit can be used for both fresh tissue and frozen material.The ROTI^®Quick Kit is based on the GITC isolation method by Chomczynski and Sacchi (Chomczynski and Sacchi. (1987) Anal. Biochem. 162:156) and includes three optimised ready-to-use solutions that were developed by molecular biologists and have proven effective for many years.After the tissue has been homogenised, the RNA is purified in a single step and isolated by precipitation. Each isolation takes approximately 2,5 hours, and multiple isolations can easily be performed simultaneously. The incubation and centrifuging steps take about 2 hours.

Preparation as given in the instruction-for-use is optimised for RNA isolation from approx. 0,2 g of tissue or cell material. The amount of material isolated per batch may easily be up- or downscaled to larger or smaller amounts of tissue.The isolated total RNA is highly pure and may be used directly for all common down-stream applications, such as RT-PCR, Northern blotting and reverse transcription. Detailed instructions-for-use are enclosed with each kit.

ROTI^®Quick Kit 1, ROTI^®Quick Kit 2 and ROTI^®Quick Kit 3.Contents of this Kit may not be bought separately.