Vivacon® 500 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations. 1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62. Concentration of up to 0.5 ml genomic DNA or peptide samples Low binding material Reverse spin possibility MWCO printed on device Complete and highly reproducible concentrate recovery Selected cut offs available sterile and DNA free* for e.g. forensic applications * qPCR assay over 55 cycles with a verified limit of detection < 2 pg Performance Characteristics for DNA Start volume 0.5 ml, sample concentration 50 ng/ml.

	Sample size (bp)	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %	g-force (xg)
2,000 MWCO	10	60 min.	93 %	7,500
10,000 MWCO	30	25 min.	94 %	7,500
30,000 MWCO	50	18 min.	88 %	5,000
50,000 MWCO	300	18 min.	91 %	5,000
100,000 MWCO	600	10 min.	87 %	3,000
125,000 MWCO	650	12 min.	85 %	2,000
125,000 MWCO	900	9 min.	94 %	3,000

Performance Characteristics for Proteins Start volume 0.5 ml, sample and concentration of proteins as specified in table.

	Sample	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %	g-force (xg)
2,000 MWCO	0.25 mg/ml cytochrome c	30 min.	95 %	14,000
10,000 MWCO	0.25 mg/ml cytochrome c	15 min.	92 %	14,000
30,000 MWCO	1.0 mg/ml BSA	10 min.	95 %	14,000
50,000 MWCO	1.0 mg/ml BSA	10 min.	92 %	14,000
100,000 MWCO	1.0 mg/ml bovine IgG	11 min.	90 %	8,000
125,000 MWCO	1.0 mg/ml bovine IgG	10 min.	81 %	8,000