.

The optimal membrane for DNA/RNA transfer, especially colony and plaque lifting and all multiple rehybridisations.ROTI^®Nylon 0.45 is a neutral, reinforced nylon membrane. Like all ROTI^®Nylon membranes, it has a very high tensile strength and is highly resistant to alcohols, acetone and many other organic solvents.ROTI^®Nylon 0.45 is not charged at buffer pH 6.5 and has excellent hydrophilic properties due to the amino and carboxy groups on the surface. The membrane charge (ζ-potential) can be adjusted by the pH value of the buffer system (positive membrane charge at pH<6.5; negative membrane charge at pH>6.5). Steam sterilisation is possible.The covalent binding of transferred nucleic acid is achieved by crosslinking (UV crosslinking for 20 s at 260 nm). The excellent signal/background ratio of the membrane then enables highly sensitive detections (single copy gene detection) and multiple rehybridisations without loss of signal quality.