Vivaspin® centrifugal concentrators are disposable ultrafiltration devices for the concentration of biological samples. Vivaspin® 500 centrifugal filter units offer a simple, one step procedure for sample concentration with starting volumes up to 500 µl. They can effectively be used in fixed angle rotors accepting 2.2 ml centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations. Vivaspin® 500 units are available with PES membrane in a wide range of molecular weight cut-offs. Concentrate recoveries of > 90% can be reached in less than 30 min. The dead stop pocket prevents concentration to dryness. High flux PES membrane High retentate recovery > 90 % Vertical membrane design for ultra fast concentration Universal rotor compatibility Safe sample concentration due to dead-stop volume Convenient concentrate recovery by pipetting Volume graduation for easy reading/monitoring Vivaspin® 500 Polyethersulfone, 10 kDa Performance Characteristics

	Time to concentrate up to 30x [min.] at 20°C	Concentrate recovery %
Start volume	500 µl	500 µl
Aprotinin 0.25 mg/ml (6,500 MW)
3,000 MWCO PES	30	96 %
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES	15	96 %
10,000 MWCO PES	5	96 %
30,000 MWCO PES	5	95 %
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES	10	96 %
50,000 MWCO PES	10	96 %
100,000 MWCO PES	10	96 %