Rad17 (phospho Ser646) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES1536-50, ES1536-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h

Alternate Name: RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog

Source: Rabbit

Applications: WB; ELISA; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Rad17 (phospho Ser646)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Human Gene ID: 5884

Human SWISS Prot NO: O75943

Subcellular Location: Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.

Research Use Only